       Document 0798
 DOCN  M9590798
 TI    Cellular CD44S as a determinant of human immunodeficiency virus type 1
       infection and cellular tropism.
 DT    9509
 AU    Dukes CS; Yu Y; Rivadeneira ED; Sauls DL; Liao HX; Haynes BF; Weinberg
       JB; Department of Medicine, Veterans Affairs Medical Center, Durham,;
       North Carolina 27705, USA.
 SO    J Virol. 1995 Jul;69(7):4000-5. Unique Identifier : AIDSLINE
       MED/95287447
 AB    CD4 is the predominant cell membrane protein that binds human
       immunodeficiency virus type 1 (HIV-1) gp120 and facilitates HIV-1
       infection, but other membrane-associated molecules may be involved in
       determining HIV-1 cellular infection. Our prior work had suggested that
       CD44, the transmembrane receptor for hyaluronan, might play a role in
       the infection of mononuclear phagocytes with HIV-1. In the present work,
       we have used cells of the CD4-positive, CD44-negative human
       T-lymphoblast cell line Jurkat to study the role of CD44 in HIV-1
       infection and tropism. Cells were transfected with cDNA for the standard
       (S, or hematopoietic) CD44 isoform CD44S or the epithelial isoform
       CD44E. The resultant lines expressed appropriate CD44S or CD44E mRNA and
       protein. While the parent Jurkat cells, those transfected with vector
       alone, and those transfected with CD44E could be productively infected
       with only the lymphocytotropic strain HIV-1-LAI, cells transfected with
       CD44S were rendered susceptible to productive infection with the
       monocytotropic strains HIV-1-BaL and HIV-1-ADA. Also, CD44S-transfected
       cells displayed higher levels of infection with HIV-1-LAI than did the
       other transfected Jurkat cells. The transfected cell line cells all had
       comparable growth rates and expressed similar levels of the membrane
       antigens CD4, CD7, major histocompatibility complex (MHC) class I, MHC
       class II, and CD11a, while levels of CD3 were slightly higher in cells
       transfected with vector alone and in one of the clones transfected with
       CD44S. Hyaluronan binding was increased in cells transfected with either
       CD44S or CD44E. Mouse NIH 3T3 fibroblasts transfected with human CD4,
       human CD44S, or both human CD4 and CD44S displayed the appropriate
       antigens, but they could not be productively infected with
       lymphocytotropic or monocytotropic strains of HIV-1. The results
       indicate that in human leukocytes, CD44S is an important determinant of
       HIV-1 productive infection and may be involved in viral cellular
       tropism.
 DE    Antigens, CD4/PHYSIOLOGY  Base Sequence  Carrier Proteins/*PHYSIOLOGY
       Cell Line  Human  HIV-1/*PHYSIOLOGY  Molecular Sequence Data  Receptors,
       Cell Surface/*PHYSIOLOGY  Receptors, Lymphocyte Homing/*PHYSIOLOGY
       Support, Non-U.S. Gov't  Support, U.S. Gov't, Non-P.H.S.  Support, U.S.
       Gov't, P.H.S.  Transfection  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

