       Document 0818
 DOCN  M9590818
 TI    A modified ELISA and western blot accurately determine anti-human
       immunodeficiency virus type 1 antibodies in oral fluids obtained with a
       special collecting device.
 DT    9509
 AU    Emmons WW; Paparello SF; Decker CF; Sheffield JM; Lowe-Bey FH; HIV
       Evaluation Unit, National Naval Medical Center, Bethesda,; Maryland,
       USA.
 SO    J Infect Dis. 1995 Jun;171(6):1406-10. Unique Identifier : AIDSLINE
       MED/95287029
 AB    Serum and saliva from 195 known human immunodeficiency virus
       (HIV)-seropositive patients and 198 military personnel undergoing annual
       HIV serologic testing were evaluated in a prospective, blinded fashion
       for anti-HIV-1 antibodies. Oral specimens, collected with a device
       designed to concentrate oral mucosal transudate from whole saliva, were
       tested by a modified ELISA and by Western blot. Serum was tested in a
       standard manner. All 195 HIV-1-seropositive subjects had detectable
       anti-HIV-1 antibodies in their saliva by ELISA; 190 saliva samples were
       positive by Western blot and 5 were indeterminate. None of the 198
       military personnel were positive by ELISA of serum or oral fluid. The
       sensitivity, specificity, and positive and negative predictive values
       for ELISA of saliva were each 100%. The serologic testing of oral
       mucosal transudate appears to be a simple, safe, sensitive, and specific
       method for detecting anti-HIV-1 antibodies.
 DE    Adult  Blotting, Western/*METHODS  Enzyme-Linked Immunosorbent
       Assay/*METHODS  Female  Human  HIV Antibodies/*ANALYSIS  HIV
       Infections/*DIAGNOSIS  HIV-1/*IMMUNOLOGY  Male  Mouth Mucosa/*IMMUNOLOGY
       Prospective Studies  Saliva/*IMMUNOLOGY  Support, U.S. Gov't, Non-P.H.S.
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

