       Document 0821
 DOCN  M9590821
 TI    In vivo activity and in vitro specificity of CD4+ Th1 and Th2 cells
       derived from the spleens of diabetic NOD mice.
 DT    9509
 AU    Healey D; Ozegbe P; Arden S; Chandler P; Hutton J; Cooke A; Department
       of Pathology, Cambridge University, United Kingdom.
 SO    J Clin Invest. 1995 Jun;95(6):2979-85. Unique Identifier : AIDSLINE
       MED/95286868
 AB    CD4+ T cell lines were generated from the spleens of diabetic NOD mice
       against crude membrane preparations derived from a rat insulinoma.
       Adoptive transfer of these lines into neonatal mice confirms that overt
       diabetes is induced by gamma-IFN-secreting Th1 cells, whereas transfer
       of IL-4-secreting Th2 cells resulted in a nondestructive peri-islet
       insulitis. Analysis of the antigens recognized by individual T cell
       clones from the Th1 line included reactivity against an insulinoma
       membrane fraction enriched in proteins of approximately 38 kD. Immune
       responses to the same antigen preparation have been associated with T
       cell clones derived from human insulin-dependent diabetes mellitus. The
       specificity of Th2 cells includes reactivity to a fraction enriched in
       proteins of 30 kD. The data suggest that in insulin-dependent diabetes
       mellitus the balance between beta cell destruction, associated with
       intra-islet infiltration, and nondestructive (potential protective)
       peri-islet insulitis may depend on both the antigens recognized, and the
       prevailing cytokine environment.
 DE    Animal  Autoantigens/*CHEMISTRY/IMMUNOLOGY  CD4-Positive
       T-Lymphocytes/*IMMUNOLOGY  Diabetes Mellitus,
       Insulin-Dependent/*IMMUNOLOGY  Immunity, Cellular  Islets of
       Langerhans/*IMMUNOLOGY  Mice  Mice, Inbred NOD/*IMMUNOLOGY  Molecular
       Weight  Proteins/CHEMISTRY/IMMUNOLOGY  Spleen/CYTOLOGY  Support,
       Non-U.S. Gov't  Th1 Cells/*IMMUNOLOGY  Th2 Cells/*IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

