       Document 1160
 DOCN  M9591160
 TI    Diagnosis of intestinal microsporidiosis by examination of stool and
       duodenal aspirate with Weber's modified trichrome and Uvitex 2B strains.
 DT    9509
 AU    DeGirolami PC; Ezratty CR; Desai G; McCullough A; Asmuth D; Wanke C;
       Federman M; Department of Pathology, New England Deaconess Hospital,
       Boston,; Massachusetts 02115, USA.
 SO    J Clin Microbiol. 1995 Apr;33(4):805-10. Unique Identifier : AIDSLINE
       MED/95310442
 AB    Severe, chronic diarrhea is a frequent complication of human
       immunodeficiency virus disease, and intestinal microsporidiosis is being
       recognized with increasing frequency in patients with AIDS. Noninvasive,
       cost-effective techniques are needed to optimize its diagnosis. Weber's
       modified trichrome stain (MTS) and the fluorochrome Uvitex 2B stain were
       used to detect microsporidial spores in smears of stool and duodenal
       aspirate (DA) samples received from human immunodeficiency
       virus-infected patients for examination for ova and parasites. Of 305
       samples (292 stool and 13 DA samples) from 140 patients examined by MTS,
       83 samples from 26 (18.6%) of the patients were positive for
       microsporidia (23 patients diagnosed initially and 3 diagnosed upon
       review). A subset of the samples studied by MTS consisting of 108 smears
       of stool and DA specimens from 60 patients was examined by Uvitex 2B.
       All 44 samples positive by MTS were also positive by Uvitex 2B. In
       addition, seven specimens and three patients were initially detected as
       positive by Uvitex 2B only (all three patients were positive also by MTS
       upon review). Confirmation of the diagnosis was obtained for 24 of 26
       smear-positive patients by duodenal biopsy and/or stool transmission
       electron microscopy. Of 114 patients with stained smears negative for
       microsporidia, 23 had duodenal biopsies which showed no microsporidia.
       For the 43 patients who underwent duodenal biopsy, the sensitivity of
       both the MTS and the Uvitex 2B methods compared with biopsy results was
       100%. Of six patients with negative duodenal biopsies and positive
       stained smears, four had microsporidia demonstrated by stool
       transmission electron microscopy.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Animal  AIDS-Related Opportunistic Infections/COMPLICATIONS/*DIAGNOSIS/
       PARASITOLOGY  Duodenum/PARASITOLOGY  Feces/PARASITOLOGY  Female  Human
       Intestinal Diseases, Parasitic/COMPLICATIONS/*DIAGNOSIS/  PARASITOLOGY
       Male  Microscopy, Electron  Microspora
       Infections/COMPLICATIONS/*DIAGNOSIS/PARASITOLOGY
       *Microsporida/CLASSIFICATION/ISOLATION & PURIF/ULTRASTRUCTURE  Parasite
       Egg Count  Stains and Staining/METHODS  Suction  CLINICAL TRIAL  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

