       Document 1170
 DOCN  M9591170
 TI    Human peripheral blood CD4+ and CD8+ T cells express Th1-like cytokine
       mRNA and proteins following in vitro stimulation with heat-inactivated
       Brucella abortus.
 DT    9509
 AU    Zaitseva MB; Golding H; Betts M; Yamauchi A; Bloom ET; Butler LE; Stevan
       L; Golding B; Laboratory of Retrovirus Research, U.S. Food and Drug;
       Administration, Bethesda, Maryland 20892, USA.
 SO    Infect Immun. 1995 Jul;63(7):2720-8. Unique Identifier : AIDSLINE
       MED/95310033
 AB    Defining the pattern of lymphokine production associated with Brucella
       abortus is critical for advancing the development of B. abortus as a
       vaccine carrier. In the present study we investigated the ability of
       heat-inactivated B. abortus or lipopolysaccharide from B. abortus to
       induce lymphokine production from purified human T cells in vitro. Gamma
       interferon (IFN-gamma), interleukin-2 (IL-2), IL-4, and IL-5 induction
       was assayed by mRNA-specific PCR and by enzyme-linked immunosorbent
       assay and bioassay for protein production. Following depletion of
       monocytes and B cells, B. abortus increased IFN-gamma and IL-2 mRNA
       expression in purified T cells compared with expression in unstimulated
       cells. In contrast, no IL-5 mRNA expression and only transient low-level
       IL-4 mRNA expression and no IL-4 protein secretion were detected.
       Phytohemagglutinin or phorbol myristate acetate plus ionomycin induced
       mRNA and protein for all these cytokines. Similar results were obtained
       with LPS purified from B. abortus. Removal of NK cells did not reduce
       lymphokine production, and enriched NK cells did not express IFN-gamma
       mRNA or secrete IFN-gamma protein in response to B. abortus, indicating
       that NK cells were not the responding population. Both CD4+ and CD8+
       populations produced IFN-gamma and IL-2 in response to B. abortus.
       Preincubation of resting T cells with B. abortus or LPS from B. abortus
       for 7 days induced their differentiation into Th1-like cells as judged
       by their subsequent lymphokine response to phorbol myristate acetate
       plus ionomycin. These results suggest that B. abortus can induce
       differentiation of Th0 into Th1-type cells.
 DE    Base Sequence  Brucella abortus/*IMMUNOLOGY  Cytokines/*METABOLISM
       CD4-Positive T-Lymphocytes/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  DNA Primers/CHEMISTRY  Gene Expression/DRUG
       EFFECTS  Heat  Human  Interferon Type II/SECRETION
       Interleukin-2/GENETICS  Interleukin-4/BIOSYNTHESIS
       Ionomycin/PHARMACOLOGY  Killer Cells, Natural/*IMMUNOLOGY
       Lipopolysaccharides/IMMUNOLOGY  Molecular Sequence Data  RNA,
       Messenger/GENETICS  T-Lymphocyte Subsets/*IMMUNOLOGY
       Tetradecanoylphorbol Acetate/PHARMACOLOGY  Th1 Cells/*IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

