       Document 0211
 DOCN  M95A0211
 TI    Growth-deficient mycobacteria in patients with AIDS: diagnosis by
       analysis of DNA amplified from blood or tissue.
 DT    9510
 AU    Emler S; Bottger EC; Broers B; Cassis I; Perrin L; Hirschel B; Division
       des Maladies Infectieuses, Hopital Cantonal; Universitaire de Geneve,
       Switzerland.
 SO    Clin Infect Dis. 1995 Apr;20(4):772-5. Unique Identifier : AIDSLINE
       MED/95315374
 AB    Amplification and sequencing of mycobacterial ribosomal RNA genes (16S
       rDNA) may permit the detection of growth-deficient species (i.e., those
       exhibiting no growth or those whose growth is delayed for more than 12
       weeks). Of blood samples from 26 patients with AIDS and a liver sample
       from one additional AIDS patient, three samples (two of blood and the
       one of liver) were positive by polymerase chain reaction only; cultures
       of these three samples remained negative for more than 12 weeks.
       Analysis of amplified 16S rDNA from blood revealed a sequence
       characteristic of Mycobacterium genavense in the first case, in which
       one of many previous blood cultures had also been positive for M.
       genavense. The sequences found in the second and third cases were
       characteristic of Mycobacterium avium. The sample from the second
       patient was a liver biopsy specimen in which acid-fast bacilli were
       visualized; the culture of this specimen yielded M. avium after 7
       months. The third sample was a blood sample from a patient in whom a
       relapse of treated M. avium infection was suspected. These results
       indicate that amplification and sequencing of mycobacterial 16S rDNA may
       permit early diagnosis and provide a rationale for treatment of
       infections due to growth-deficient mycobacteria.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/*MICROBIOLOGY  Adult  Base
       Sequence  Case Report  DNA, Bacterial/*ANALYSIS  Female  Human  Male
       Middle Age  Molecular Sequence Data  Mycobacterium/*GROWTH &
       DEVELOPMENT/GENETICS/ISOLATION & PURIF  Polymerase Chain Reaction
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

