       Document 0263
 DOCN  M95A0263
 TI    Inhibition of human immunodeficiency virus expression by sense
       transcripts encoding the retroviral leader RNA.
 DT    9510
 AU    Berkhout B; van Wamel JL; Department of Virology, Academic Medical
       Center, University of; Amsterdam, The Netherlands.
 SO    Antiviral Res. 1995 Mar;26(2):101-15. Unique Identifier : AIDSLINE
       MED/95328855
 AB    Towards gene therapy for the treatment of human immunodeficiency virus
       type 1 (HIV-1) infections, we tested the potency of several antiviral
       constructs in transient HIV-1 production assays. Whereas little effect
       was obtained with antisense- and TAR decoy-constructs, we measured
       efficient inhibition of HIV-1 mRNA translation and virion production in
       the presence of HIV-1 leader-containing transcripts. The infectivity of
       these virions was also reduced by this sense inhibitor RNA. These
       results suggest that leader-encoded functions, like the dimer-linkage
       structure, can be used to specifically inhibit HIV expression in trans.
 DE    Antiviral Agents/*PHARMACOLOGY  Blotting, Northern  *Gene Expression
       Regulation, Viral  Hela Cells  Human  HIV Long Terminal Repeat/*GENETICS
       HIV-1/*DRUG EFFECTS/PHYSIOLOGY  Retroviridae Proteins/GENETICS
       RNA-Binding Proteins/GENETICS  RNA, Antisense/PHARMACOLOGY  RNA,
       Viral/*PHARMACOLOGY  Support, Non-U.S. Gov't  Transcription, Genetic
       Virus Replication/DRUG EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

