       Document 0063
 DOCN  M95B0063
 TI    A nuclear factor (NF2d9) that binds to the male-specific P450 (Cyp 2d-9)
       gene in mouse liver.
 DT    9511
 AU    Sueyoshi T; Kobayashi R; Nishio K; Aida K; Moore R; Wada T; Handa H;
       Negishi M; Pharmacogenetics Section, National Institute of
       Environmental; Health Sciences, Research Triangle Park, North Carolina
       27709,; USA.
 SO    Mol Cell Biol. 1995 Aug;15(8):4158-66. Unique Identifier : AIDSLINE
       GENBANK/U20122
 AB    Expression of the Cyp 2d-9 (steroid 16 alpha-hydroxylase) gene in mouse
       liver is male specific in such Mus musculus domesticus strains as FVB/N,
       whereas the corresponding P450 genes in the wild mouse species Mus
       spretus are not sex specific in their expression. These parental
       differences in the gene expressions were independently inherited in F1
       offspring from crosses of FVB/N and M. spretus. A 5' flanking sequence
       (-110CTC CTCCCTATTCCGGGCC-92) was defined as a regulatory element (named
       SDI-A1) for the domestic Cyp 2d-9 promoter. The nucleotide which
       corresponds to T at position -99 within SDI-A1 was found to be
       substituted with C in the wild mouse P450 genes. The placing of C at
       position -99 abolished the transcriptional activity of SDI-A1 in HepG2
       cells as well as the binding of SDI-A1 to a nuclear factor. This factor
       (designated NF2d9) was purified from mouse nuclear extracts, and its
       cDNA cloned. The purified NF2d9 bound to SDI-A1 but not to the mutated
       SDI-A1 with C at position -99. The deduced amino acid sequence revealed
       that NF2d9 is 72 and 94% identical to mouse CP2 and human LBP-1a,
       respectively. NF2d9 thus belongs to the CP2 family and is the mouse
       homolog of human LBP-1a, which modulates human immunodeficiency virus
       type 1 transcription. Anti-NF2d9, which was raised against the
       bacterially expressed protein, supershifted the SDI-A1 complex with the
       liver nuclear extract. Both the bacterially expressed and in
       vitro-translated NF2d9 inhibited SDI-A1 complex formation, although they
       did not bind to SDI-A1 directly. The results, therefore, indicate that
       the domestic Cyp 2d-9 gene can be regulated through a specific
       association of NF2d9 with SDI-A1.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cell Nucleus/METABOLISM
       Cloning, Molecular  Crosses, Genetic  Cytochrome P-450/*GENETICS
       DNA-Binding Proteins/*GENETICS/IMMUNOLOGY/ISOLATION & PURIF/  METABOLISM
       Escherichia coli/GENETICS  Gene Expression Regulation, Enzymologic
       Liver/*METABOLISM  Male  Molecular Sequence Data  Muridae  Mutation
       Phylogeny  Protein Binding  Recombinant Fusion
       Proteins/IMMUNOLOGY/METABOLISM  Regulatory Sequences, Nucleic
       Acid/GENETICS  Sequence Analysis, DNA  *Sex Characteristics  Steroid
       Hydroxylases/*GENETICS  Tissue Distribution  *Transcription, Genetic
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

