       Document 0128
 DOCN  M95B0128
 TI    Shigella infection induces cellular activation of T and B cells and
       distinct species-related changes in peripheral blood lymphocyte subsets
       during the course of the disease.
 DT    9511
 AU    Islam D; Bardhan PK; Lindberg AA; Christensson B; Division of Clinical
       Bacteriology, Karolinska Institute, Huddinge; University Hospital,
       Sweden.
 SO    Infect Immun. 1995 Aug;63(8):2941-9. Unique Identifier : AIDSLINE
       MED/95347806
 AB    Immunophenotypic changes in peripheral blood lymphocytes (T, B, and NK
       cells) in patients during shigellosis was characterized by using
       triple-color flow cytometry. Eleven Shigella dysenteriae 1-infected
       adult patients (SDIP), 11 Shigella flexneri-infected adult patients
       (SFIP), 15 age- and sex-matched healthy controls from Bangladesh (C-B),
       and 15 healthy volunteers from Sweden (V-S) were studied. In SDIP and
       SFIP, a significant increase in the CD45RO+ cells in both CD4+ and CD8+
       T cells were seen. We found evidence for sequential T-cell activation,
       as shown by increased proportions of CD25 and CD4+ cells; HLA-DR and
       CD38 on CD8+ cells, and CD54 on CD4+ and CD8+ cells. We found
       differences in the lymphocyte activation and subset patterns related to
       the infecting Shigella species. Thus, a decrease in CD45 expression was
       seen in SFIP; this decrease progressed further during the disease. The
       proportions of NK cells (CD56+ cells) and CD3- CD8+ cells out of the
       total CD8+ cells were increased in SFIP but not in SDIP. The CD3+ CD8+
       CD57+ T-cell subset was significantly lower in SDIP than in C-B. The
       proportion of B-lymphocyte-expressing activation markers CD80 and CD23
       was higher in patients than in C-B. There was a significant increase in
       the proportion of CD4+ T cells and a significant decrease in the
       percentages of total B cells, the CD3+ CD8+ CD57+ T-cell subset, and the
       CD56+ CD16+ NK-cell subset for V-S compared with C-B. Our results
       indicate that distinct subset changes and activation patterns are
       elicited in SDIP compared with SFIP and also that the degree of
       activation is related to disease severity. In addition, a common
       sequence of cell activation was seen during the disease course. The
       difference in the subset patterns seen in C-B and V-S may be related to
       differences in the levels or spectra of infectious agents in the
       environment.
 DE    Adult  Antigens, CD/METABOLISM  Antigens, Differentiation/METABOLISM
       B-Lymphocytes/*IMMUNOLOGY  CD4-Positive T-Lymphocytes/IMMUNOLOGY
       CD8-Positive T-Lymphocytes/IMMUNOLOGY  Dysentery, Bacillary/*IMMUNOLOGY
       Human  HLA-DR Antigens/METABOLISM  Intercellular Adhesion
       Molecule-1/METABOLISM  Killer Cells, Natural/IMMUNOLOGY  Lymphocyte
       Count  Lymphocyte Subsets/*IMMUNOLOGY  *Lymphocyte Transformation  Male
       Nucleosidases/METABOLISM  Shigella dysenteriae/*IMMUNOLOGY  Shigella
       flexneri/*IMMUNOLOGY  Support, Non-U.S. Gov't  T-Lymphocytes/*IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

