       Document 0177
 DOCN  M95B0177
 TI    Molecular analysis of clonality in Castleman's disease.
 DT    9511
 AU    Soulier J; Grollet L; Oksenhendler E; Miclea JM; Cacoub P; Baruchel A;
       Brice P; Clauvel JP; d'Agay MF; Raphael M; et al; Laboratoire
       d'Hematologie Moleculaire, Hopital de Jour; Hematologie-Oncologie,
       Paris, France.
 SO    Blood. 1995 Aug 1;86(3):1131-8. Unique Identifier : AIDSLINE
       MED/95345464
 AB    Castleman's disease (CD) is a rare atypical lymphoproliferative disorder
       that is morphologically and clinically heterogenous and is associated
       with a risk of developing malignant lymphoma. We report the clonality
       status of CD tissues in 34 patients, including 14 patients infected by
       the human immunodeficiency virus (HIV). Four patients presented a
       localized form and 30 presented a multicentric form. Two cases were
       associated with B-cell lymphoma, 3 cases with Hodgkin's disease, and 9
       cases (8 HIV+) with Kaposi's sarcoma. Histologically, 8 cases were of
       the hyaline-vascular type and 26 were of the plasma cell or mixed types.
       The Ig and T-cell receptor (TCR) V(D)J rearrangements were analyzed
       using polymerase chain reaction and Southern blot. Clonal IgH
       rearrangements were detected in only 4 cases, ie, 2 associated with
       B-cell lymphoma, 1 with Hodgkin's disease, and 1 case without
       malignancy. A TCR gamma rearrangement of restricted junctional size was
       amplified in 1 HIV+ case. Finally, polyclonal VH-JH and V gamma-J gamma
       rearrangements were detected in the large majority of the cases,
       irrespective of pathologic subtypes, clinical forms, and HIV status. The
       lymphoid component in CD is therefore commonly reactive, and the rare
       occurrence of detectable monoclonal lymphoid contingents may be caused
       by secondary molecular events.
 DE    Adult  Aged  B-Lymphocytes/*CYTOLOGY  Child  Clone Cells  Female  Genes,
       Immunoglobulin  Giant Lymph Node Hyperplasia/*PATHOLOGY  Human  HIV
       Infections/COMPLICATIONS  Immunoglobulin Variable Region/GENETICS
       Immunoglobulins, Heavy-Chain/GENETICS  Lymph Nodes/PATHOLOGY  Male
       Middle Age  Receptors, Antigen, T-Cell, gamma-delta/GENETICS  Support,
       Non-U.S. Gov't  T-Lymphocytes/*CYTOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

