       Document 0256
 DOCN  M95B0256
 TI    Contribution of polymerase chain reaction and radioimmunoprecipitation
       assay in the confirmation of human T-lymphotropic virus infection in
       French blood donors. Retrovirus Study Group of the French Society of
       Blood Transfusion.
 DT    9511
 AU    Defer C; Coste J; Descamps F; Voisin S; Lemaire JM; Maniez M; Courouce
       AM; Centre Regionaux de Transfusion Sanguine, Lille, France.
 SO    Transfusion. 1995 Jul;35(7):596-600. Unique Identifier : AIDSLINE
       MED/95357884
 AB    BACKGROUND: To verify the criteria for human T-lymphotropic virus (HTLV)
       seropositivity in Western blot (WB) proposed by the Retrovirus Study
       Group of the French Society of Blood Transfusion, 186 blood donations
       that were repeatedly reactive in HTLV enzyme-linked immunosorbent assay,
       selected according to their WB pattern, were tested by polymerase chain
       reaction (PCR) and radioimmunoprecipitation assay (RIPA). STUDY DESIGN
       AND METHODS: In two commercially available WBs, 12 samples were
       confirmed as positive (rgp21+p19+p24) and 174 were interpreted as
       indeterminate (one or two reactivities to these proteins). The primer
       pairs used for the PCR allowed the amplification of type I (HTLV-I) or
       type II (HTLV-II) (or both) sequences. The RIPA was performed with two
       35S-labeled cell lines: HTLV-I infected HUT 102/B2 and HTLV-II-infected
       MoT. RESULTS: Of the 12 positive samples, 11 were classified as
       HTLV-I-positive and one as HTLV-II-positive. Among the 174 indeterminate
       samples, three (WB pattern: rgp21+, p19+, p24-) were HTLV-I positive in
       PCR (one of them was positive in RIPA also); the other 171 were HTLV
       negative. CONCLUSION: In the study of a population in which 97 percent
       of HTLV infections are due to HTLV-I, these data support the
       three-protein criteria (rgp21, p19, and p24) for a positive blot
       reading. No HTLV infection was observed when rgp21 did not react.
       Consequently, p19 and/or p24 band patterns represent false reactivity
       and do not require PCR or RIPA confirmation. To discriminate between
       false- and true-positive results in the absence of MTA-1 or K55
       reactivity, PCR and/or RIPA is required only when rgp21 reactivity is
       associated with one gag band (p19 or p24).
 DE    Base Sequence  *Blood Donors  France  Human  HTLV-I/*ISOLATION & PURIF
       HTLV-I Infections/*DIAGNOSIS  Molecular Sequence Data  Polymerase Chain
       Reaction/METHODS  Radioimmunoassay/METHODS  Support, Non-U.S. Gov't
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

